Fluorescence study of dyes

Title: Fluorescence study of dyes
Category: /Science & Technology
Details: Words: 838 | Pages: 3 (approximately 235 words/page)
Fluorescence study of dyes
Fluorescence Study of the Kinetics of Energy Transfer Between Dyes 1. Introduction Coumarin 1 and Sodium fluorescein are two dyes which absorb and emit light in the visible region. By using a spectrophotometer we are recording an absorption spectrum and then determining the molar decadic absorption coefficients, which will be used later to interpret and analyse the fluorescence spectra. For fluorescence spectra, there are two kinds, the excitation and the emission spectrum. The excitation spectrum is obtained …showed first 75 words of 838 total…
You are viewing only a small portion of the paper.
Please login or register to access the full copy.
…showed last 75 words of 838 total…gives us the following graph. Values obtained: JDA = 6.897x1031 nm mol -1 R0 = 0.14 nm (R0)eff = 0.584 nm based on equation (A3-13) So we see that those are very clearly quite different, and that could show that it does not obey to Forster's theory. 3. Conclusion So we have shown that the quenching of Coumarin 1 by Sodium fluorescein is obeyed by Stern-Volmer kinetics, that there is mainly dipole-dipole transfer, but could not agree with Forster's theory.

Need a custom written paper?
Buy a custom written essay and get 20% OFF the first order